Artikelnummer | DIA-CAL-250 |
---|---|
Spezifität | mutiertes Calreticulin / CALR |
Spezies-Reaktivität | Human |
Immunogen | C-Neoterminus von mutiertem CALR |
Wirtsspezies | Maus |
Isotyp | IgG2a |
Klon | CAL2 |
Klonalität (Mono-/Polyklonal) | monoklonal |
Anwendung | Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe) |
Konjugation | unkonjugiert |
Verdünnung | Histo-/Zytochemie 1:50 – 1:100 |
Format | 0,05% NaN3, 2% BSA, gereinigter Antikörper (aus Kulturüberstand), in PBS (pH 7,4), Lyophilisat |
Produktlinie / Thema | Tumormarker/-biologie |
Zweckbestimmung | nur für Forschungszwecke |
Temperatur - Lagerung | 2-8°C |
Temperatur - Transport | bei Raumtemperatur |
Suchcode | Calreticulin Mutation, FFPE, Paraffin |
Hersteller / Marke | dianova |
Uniprot_ID | P27797 |
Gene_ID | 811 |
Alias | Autoantigen Ro, CALR, Calregulin, Calreticulin, CC1qR, CRP55, CRT, CRTC, CTR, Endoplasmic reticulum resident protein 60, ERp60, grp60, HACBP, RO, Sicca Syndrome Antigen A |
CAL2 labels the megakaryocytes in myeloproliferative neoplasms (essential thrombocythaemia (ET) and primary myelofibrosis (PMF)) with CALR mutation and enables to distinguish ET and PMF with CALR mutation from polycythemia vera (PV), from CALR mutation negative ET and PMF and from reactive bone marrow.
All types of CALR mutation result in a novel C-terminus. This harbors a common epitope expressed in all kinds of CALR mutations. The CAL2 antibody is directed against this common epitope. Therefore, it can be concluded that the CAL2 antibody is able to detect all CALR mutations.
A and C: Selective staining of mutated CALR protein in megakaryocytes of two PMF cases, respectively in prefibrotic phase and in fibrotic phase, in which Sanger sequencing detected a CALR mutation.
B and D: Absent CAL2 staining in two PMF cases, respectively in prefibrotic and in fibrotic phase, both without molecularly detected mutated CALR. The fibrotic stroma remains unstained (C and D).
A
B
C
D
dianova
自1982年以来,我们为研究和常规客户提供了高质量的二级抗体、初级抗体、免疫卫星和免疫化学探测系统以及其他生命科学产品。
自1982年成立以来,dianova GmbH为生命科学专业人士提供抗体,免疫测定和分子生物学产品。30多年的专业知识使人们对生化应用有了基本的了解,并对大学,医院和制药行业的研究和常规诊断实验室对客户需求的深刻理解。
品牌 | 货号 | 名称 | 规格 |
迪亚诺娃 | DGHG-B001 | Ziege抗人类IgG(H + L)-金40nm OD15, | 1毫升 |
迪亚诺娃 | DGHG-B009 | Ziege抗人类IgG(H + L)-金40nm OD15, | 9毫升 |
迪亚诺娃 | DGHA-B001 | 山羊抗人金40nm OD15, | 1毫升 |
迪亚诺娃 | DGHA-B009 | 山羊抗人IgA-金40nm OD15, | 9毫升 |
迪亚诺娃 | DGHM-B001 | 山羊抗人IgM(µ)-金40nm OD15, | 1毫升 |
迪亚诺娃 | DGHM-B009 | 山羊抗人IgM(µ)-金40nm OD15, | 9毫升 |
迪亚诺娃 | DGMG-B001 | 山羊抗小鼠IgG(H + L)-金色40nm OD15, | 1毫升 |
迪亚诺娃 | DGMG-B009 | 山羊抗小鼠IgG(H + L)-金色40nm OD15, | 9毫升 |